Home Assignment # 1- 05.19.2017

Deadline of submission: by 4 pm of 05.26.2017

Questions:

1)    Calculate the value ofc2 andassign the type of mutation from the segregation data and chi-square value

Strain/

generation

 

Female†††††††††††††††††††††††††††† †††††††††††††††††

†††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††

Male

 

Ein+

 

†† Ein-

 

c2

Type of mutation

ein4

F1

 

Wild-type

 

ein4

 

0

 

†††† 8

 

 

F2

 

 

 

13

††† 36

 

 

ein5

F1

Wild-type

ein5-1

10

†††† 0

 

 

F2

 

 

 

41

††† 13

 

 

F1

 

Wild-type†††††††††††††

ein5-2

5

†††† 0

 

 

F2

 

 

53

††† 19

 

 

ein6

F1

 

Wild-type

 

ein6

 

9

††††

††††† 0

 

 

F2

 

 

 

374

††† 38

 

 

eir1

F1††

 

Wild-type

eir1-1

19

 

††††† 0

 

 

F2

 

 

 

48

†††† 18

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2)  Write in detail about CRISPR-cas9 method. What aspects of this method are better than existing methods .

Instruction to students:

Answer the second question in your own words. Donít copy and paste from anywhere. Submit the assignment within the deadline. Late submitting or copied assignments will not be graded.