Publications

2012

Saito, H., Sawada, T., Yaegashi, T., Goto, Y., Jin, J., Sawai, K. and Hashizume, T. (2012) Kisspeptin-10 stimulates the release of luteinizing hormone and testosterone in pre- and post-pubertal male goats. Anim. Sci. J. In press.

Jin, J., Yaegashi, T., Sawai, K. and Hashizume, T. (2012)Effects of photoperiod on the secretion of growth hormone in female goats. Anim. Sci. J. In press.

Sekizawa, F., Sawai, K., Tanaka, M. and Okuda, K. (2012) Relationship between embryo collection results after superovulation treatment of Japanese Black cows and their plasma β-carotene and vitamin concentrations. J. Reprod. Dev. In press.

Sawai, K., Fujii, T., Hirayama, H., Hashizume, T. and Minamihashi, A. (2012) Epigenetic status and full-term development of bovine cloned embryos treated with trichostatin A. J. Reprod. Dev. In press.

後藤由希, 斉藤隼人, 金 金, 平田統一, 澤井 健, 橋爪 力 (2012) Kisspeptin-10の成熟雌牛の黄体形成ホルモンおよび卵胞刺激ホルモン放出作用. 東畜会報. 61: 47-54.

2011

Sawai, K., Takahashi, M., Fujii, T., Moriyasu, S., Hirayama, H., Minamihashi, A., Hashizume, T. and Onoe, S. DNA methylation status of bovine blastocyst embryos obtained from various procedures. Jour. Reprod. Devel. 57: 236-241 (2011)

Hirayama, H., Sawai, K., Hirayama, M., Hirai, T., Kageyama, S., Onoe, S., Minamihashi, A. and Moriyasu, S. Prepartum maternal plasma glucose concentration and placental glucose transporter mRNA expression in cows carrying somatic cell clone fetuses. Jour. Reprod. Devel. 57: 57-61 (2011)

Ezzat, Ahmed, A., Saito, H., Sawada, T., Yaegashi, T., Jin Jin, Sawai, K., Yamashita, T. and *Hashizume, T. Sex steroid hormones do not enhance the direct stimulatory effect of Kisspeptin-10 on secretion of growth hormone from bovine anterior pituitary cells. Anim. Sci. Jour. 82: 73-77 (2011)

Yamanaka, KI., Sakatani, M, Kubota, K., Balboula, A.Z., Sawai, K. and *Takahashi, M. Effects of Downregulating DNA Methyltransferase 1 Transcript by RNA Interference on DNA Methylation Status of the Satellite I Region and In Vitro Development of Bovine Somatic Cell Nuclear Transfer Embryos. Jour. Reprod. Devel. 57: 393-402 (2011)

澤井 健 ウシ体細胞クローン胚の遺伝子発現制御機構. 東北畜産学会報 60:74-79 (2011)

Miyashita N, Kubo Y, Yonai M, Kaneyama K, Saito N, Sawai K, Minamihashi A, Suzuki T, Kojima T, Nagai T. Cloned Cows with Short Telomeres Deliver Healthy Offspring with Normal-length Telomeres. J Reprod Dev. (2011) Aug 5. [Epub ahead of print]

2010

Kimiko Inoue, Takashi Kohda, Michihiko Sugimoto, Takashi Sado, Narumi Ogonuki1, Shogo Matoba1, Hirosuke Shiura1, Rieko Ikeda1, Keiji Mochida1, Takashi Fujii, Ken Sawai, Arie P. Otte, X Cindy Tian, Xiangzhong Yang, Fumitoshi Ishino, Kuniya Abe, Atsuo Ogura. (2010) Impeding Xist Expression from the Active X Chromosome Improves Mouse Somatic Cell Nuclear Transfer. Science, 330, 496-499.

Fujii, T., Moriyasu, S., Hirayama, H., Hashizume, T. and Sawai, K. (2010) Aberrant expression patterns of genes involved in segregation of inner cell mass and trophectoderm lineages in bovine embryos derived from somatic cell nuclear transfer. Cellular Reprogramming, 12, 617-625.

Hashizume, T., Saito, H., Sawada, T., Yaegashi, A., Ahmad, Ezzat, A., Sawai, K. and Yamashita, T. (2010)
Characteristics of stimulation of gonadotropin secretion by kisspeptin-10 in female goats. Anim. Reprod. Sci., 118, 37-41.


Sawai, K., Takahashi, M., Moriyasu, S., Hirayama, H., Minamihashi, A., Hashizume, T. and Onoe, S. (2010) Changes in the DNA methylation status of bovine embryos from the blastocyst to elongated stage derived from somatic cell nuclear transfer. Cellular Reprogramming, 12, 15-22.

Hirayama, H., Kageyama, S., Moriyasu, S., Sawai, K., Onoe, S. and Minamihashi, A. (2010) The possibility of a false positive arising from sperm DNA in the genetic diagnosis of bovine embryos. Jour. Reprod. Devel., 56, 182-186.

Ezzat, Ahmed, A., Saito, H., Sawada, T., Yaegashi, T., Jin Jin, Sawai, K., Yamashita, T. and Hashizume, T. (2010)Sex steroid hormones do not enhance the direct stimulatory effect of Kisspeptin-10 on secretion of growth hormone from bovine anterior pituitary cells. Anim. Sci. Jour., 121, 267-272.

2009

Sawai, K. (2009) Studies on gene expression in bovine embryos derived from somatic cell nuclear transfer. Jour. Reprod. Devel. 55, 11-16

Hashizume, T
., Onodera, Y., Shida, R., Isobe, E., Suzuki, S., Sawai, K., Kasuya, E. and Nagy, G.M. (2009) Characteristics of prolactin-releasing response to salsolinol (SAL) and thyrotropin-releasing hormone (TRH) in ruminants.
Domest Anim Endocrinol, 36, 99-104.

Skarzynski, D.J., Piotrowska, K.K., Bah, M.M., Korzekwa, A., Woclawek-Potocka, I., Sawai, K. and Okuda, K. (2009) Effects of exogenous tumour necrosis factor-a on the secretory function of the bovine reproductive tract depend on tumour necrosis factor-a concentrations. Reprod Dom Anim, 44, 371-379.

Ezzat, Ahmed, A., Saito, H., Sawada, T., Yaegashi, T., Yamashita, T., Hirata, T.I., Sawai, K. and Hashizume, T. (2009) Characteristics of the Stimulatory Effect of Kisspeptin-10 on the Secretion of Luteinizing Hormone, Follicle-Stimulating Hormone and Growth Hormone in Prepubertal Male and Female Cattle. Jour. Reprod. Devel., 55, 650-654

2008


Hirayama, H., Sawai, K., Moriyasu, S., Hirayama, M., Goto, Y., Kaneko, E., Miyamoto, A., Ushizawa, K., Takahashi, T. and Minamihashi, A. (2008)
Excess estrogen sulfoconjugation as the possible cause for a poor sign of parturition in pregnant cows carrying somatic cell clone fetuses.  Reproduction, 136, 639-647.

Hirayama, H., Fujikawa, A., Kageyama, S., Moriyasu, S., Sawai, K., Onoe, S. and Minamihasi, A. (2008)
Multiple genotyping in bovine pre-implantation embryos with whole genome amplification. Anim. Sci. Jour. 79: 554-560.

Saito, S., Sawai, K., Murayama, Y., Fukuda, K. and Yokoyama, K. (2008)
Nuclear transfer to study the nuclear reprogramming of human stem cells. Methods Mol. Biol. 438: 151-169.


2007


Sawai, K
.
, Kageyama, S., Moriyasu, S., Hirayama, H., Minamihashi, A. and Onoe, S. (2007)
Changes in the mRNA transcripts of insulin-like growth factor ligand, receptors, and binding proteins in bovine blastocysts and elongated embryos derived from somatic cell nuclear transfer. Jour. Reprod. Devel.53, 77-86.

Hirayama H., Katagiri S., Kageyama S., Minamihasi A., Moriyasu S., Sawai K., Onoe S. and Takahashi Y. (2007)
Rapid sex chromosomal chimerism analysis in heterosexual twin female calves by Loop-mediated Isothermal Amplification. Anim. Reprod. Sci. 101: 38-44.


2006
Kageyama, S., Hirayama, H., Moriyasu, S., Inaba, M., Ito, D., Ohta, H., Sawai, K., Minamihashi, A. and Onoe, S. (2006)

Genetic diagnosis of band 3 deficiency and sexing in bovine preimplantation embryos. Jour. Vet. Med. 68: 319-323.

Hirayama, H., Kageyama, S., Takahashi, Y., Moriyasu, S., Sawai, K., Onoe, S., Watanabe, K., Kojima, S., Notomi, T and Minamihashi, A. (2006)
Rapid sexing of water buffalo (Bubalus bubalis) embryos using loop-mediated isothermal amplification. Theriogenology 66: 1249-1256.

澤井 健(2006)
いざの扉へ―クローンとエピジェネティクスの新展開―. 丸善, 東京, pp. 26-41.


2005
Takahashi, H., Takahashi, M., Nagaya, H., Hirako, M., Sawai, K., Minamihashi, A., Inumaru, S., Yokomizo, Y., Geshi, M., Okano, A. and Okuda, K. (2005)
Establishment of a specific radioimmunoassay for bovine interferon τ. Theriogenology 63: 1050 –1060.

Sawai, K., Kageyama, S., Moriyasu, S., Hirayama, H., Minamihashi, A. and Onoe, S. (2005)
Analysis of mRNA transcripts for insulin-like growth factor receptors and binding proteins in bovine embryos derived from somatic cell nuclear transfer. Clonig and Stem Cells 7: 189-198.

Saito, S., Sawai, K., Minamihashi, A., Ugai, H., Murata, T. and Yokoyama, K. (2005)
Derivation, maintenance and differentiation of equine ES cells. Meth. Mol. Biol. 329: 59-79.
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2004以前はモー少しまって下さい!