各誌発表論文 Publication

原著論文

•Nishikawa, H., Sasaki, M., Nishiyama, K. "Membrane insertion of F0 c subunit of F0F1 ATPase depends on glycolipozyme MPIase and is stimulated by YidC."
Biochem. Biophys. Res. Commun.,487, 477-482 (2017).


•Nishiyama, K. and Tokuda, H. “Novel translocation intermediate allows re-evaluation of roles of ATP, proton motive force and SecG at the late stage of preprotein translocation”
Gene. Cells.,12, 1353-1364 (2016)


•Kumazaki, K., Chiba, S., Takemoto, M., Furukawa, A., Nishiyama, K., Sugano, Y., Mori, T., Dohmae, N., Hirata, K., Nakada-Nakura, Y. Maturana, A.D., Tanaka, Y., Mori, H., Sugita, Y., Arisaka, F., Ito, K., Ishitani, R., Tsukazaki, T.*,. Nureki, O.*.
Structural basis of Sec-independent membrane protein insertion by YidC
Nature, 509, 516-520 (2014)


•Moser, M., Nagamori, S. Huber, M., Tokuda, H. and Nishiyama, K.*
Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation
Proc. Natl. Acad. Sci. USA., 110, 9734-9739 (2013)


•Nishiyama, K.*, Maeda, M., Yanagisawa, K., Nagase, R., Komura, H., Iwashita, T., Yamagaki, T., Kusumoto, S., Tokuda, H. and Shimamoto, K.*
MPIase is a glycolipozyme essential for membrane protein integration
Nat. Commun., 3, 1260 (2012)


•Morita, K., Tokuda, H. and Nishiyama, K.*
Multiple SecA molecules drive protein translocation across a single translocon with SecG inversion
J. Biol. Chem., 287, 455-464 (2012)

•Nishiyama, K.*, Maeda, M., Abe, M., Kanamori, T., Shimamoto, K., Kusumoto, S., Ueda, T. and Tokuda, H.
A novel complete reconstitution system for membrane integration of the simplest membrane protein
Biochem. Biophys. Res. Commun. , 394, 733-736 (2010)


•Nishiyama, K.* and Tokuda, H.
Development of a functional in vitro integration system for an integral membrane protein, SecG
Biochem. Biophys. Res. Commun., 390, 920-924 (2009)

•Antonoaea, R., Furst, M., Nishiyama, K. and Muller, M.*
The periplasmic chaperone PpiD interacts with secretory proteins exiting from the SecYEG translocon
Biochemistry, 47, 5649-5656 (2008)


•Kawashima, Y., Miyazaki, E., Muller, M., Tokuda, H. and Nishiyama, K.*
Diacylglycerol specifically blocks spontaneous integration of membrane proteins and allows detection of a factor-assisted integration
J. Biol. Chem., 283, 24489-24496 (2008)


•Sugai, R., Takemae, K., Tokuda, H. and Nishiyama, K.*
Topology inversion of SecG is essential for cytosolic SecA-dependent stimulation of protein translocation
J. Biol. Chem., 282, 29540-29548 (2007)

•Nishiyama, K.*, Ikegami, A., Moser, M., Schiltz, E., Tokuda, H. and Muller, M.*
A derivative of lipid A is involved in signal recognition particle/SecYEG-dependent and -independent membrane integrations
J. Biol. Chem., 281, 35667-35676 (2006)

•Nishiyama, K.* and Tokuda, H.
Genes coding for SecG and Leu2-tRNA form an operon to give an unusual RNA comprising mRNA and a tRNA precursor
Biochim. Biophys. Acta, 1729, 166-173 (2005)


•Ikegami A., Nishiyama K., Matsuyama S. and Tokuda H.*
Disruption of rpmJ encoding ribosomal protein L36 decreases the expression of secY upstream of the spc operon and inhibits protein translocation in Escherichia coli
Biosci. Biotechnol. Biochem., 69, 1595-1602 (2005)

•Kuruma, Y., Nishiyama, K., Shimizu, Y., Muller, M. and Ueda, T.*
Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins
Biotechnol. Prog., 21, 1243-1251 (2005)


•Sugai, R., Shimizu, H., Nishiyama, K. and Tokuda, H.*
Overexpression of gnsA, a multicopy suppressor of the secG null mutation, increases acidic phospholipid contents by inhibiting phosphatidylethanolamine synthesis at low temperatures
J. Bacteriol., 186, 5968-5971 (2004)

•Kato, Y., Nishiyama, K. and Tokuda, H.*
Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction
FEBS Lett., 550, 114-118 (2003)


•Nagamori, S., Nishiyama, K. and Tokuda, H.*
Membrane topology inversion of SecG detected by labeling with a membrane-impermeable sulfhydryl reagent that causes a close association of SecG with SecA
J. Biochem., 132, 629-634 (2002)

•Sugai, R., Shimizu, H., Nishiyama, K. and Tokuda, H.*
Overexpression of yccL (gnsA) and ydfY (gnsB) increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation of Escherichia coli
J. Bacteriol., 183, 5523-5528 (2001)

•Nagamori, S., Nishiyama, K. and Tokuda, H.*
Two SecG molecules present in a single protein translocation machinery are functional even after crosslinking
J. Biochem., 128, 129-137 (2000)
(This paper received the JB award.)


•Nishiyama, K., Suzuki, H. and Tokuda, H.*
Role of the non-essential region encompassing the N-terminal two transmembrane stretches of Escherichia coli SecE
Biosci. Biotechnol. Biochem., 64, 2121-2127 (2000)


•Nishiyama, K., Fukuda, A., Morita, K. and Tokuda, H.*
Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation
EMBO J., 18, 1049-1058 (1999)


•Nishiyama, K., Furuta, M. and Tokuda, H*
Molecular cloning and functional characterization of SecE of a marine bacterium, Vibrio alginolyticus
Biochem. Biophys. Res. Commun., 251, 894-897 (1998)


•Suzuki, H., Nishiyama, K. and Tokuda, H.*
Coupled structure change of SecA and SecG revealed by the synthetic lethality of the secAcsR11 and ΔsecG::kan double mutant
Mol. Microbiol., 29, 331-341 (1998)


•Shimizu, H., Nishiyama, K. and Tokuda, H.*
Expression of gpsA encoding biosynthetic sn-glycerol 3-phosphate dehydrogenase suppresses both the LB- phenotype of a secB null mutant and the cold-sensitive phenotype of a secG null mutant
Mol. Microbiol., 26, 1013-1021 (1997)


•Kontinen, V.P., Yamanaka, M., Nishiyama, K. and Tokuda, H.*
Roles of the conserved cytoplasmic region and non-conserved carboxy-terminal region of SecE in Escherichia coli protein translocase
J. Biochem., 119, 1124-1130 (1996)


•Nishiyama, K., Suzuki, T. and Tokuda, H.*
Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation
Cell, 85, 71-81 (1996)


•Hanada, M., Nishiyama, K. and Tokuda, H.*
SecG plays a critical role in protein translocation in the absence of the proton motive force as well as at low temperature
FEBS Lett., 381, 25-28 (1996)


•Nishiyama, K., Mizushima, S. and Tokuda, H.*
Preferential interaction of SecG with SecE stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane
Biochem. Biophys. Res. Commun., 217, 217-223 (1995)


•Hanada, M., Nishiyama, K., Mizushima, S. and Tokuda, H.*
Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12)
J. Biol. Chem., 269, 23625-23631 (1994)


•Douville, K., Leonard, M., Brundage, L., Nishiyama, K., Tokuda, H., Mizushima, S. and Wickner, W.*
Band 1 subunit of Escherichia coli preprotein translocase and integral membrane export factor p12 are the same protein
J. Biol. Chem., 269, 18705-18707 (1994)


•Nishiyama, K., Hanada, M. and Tokuda, H.*
Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature
EMBO J, 13, 3272-3277 (1994)


•Nishiyama, K., Mizushima, S. and Tokuda, H.*
A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli
EMBO J., 12, 3409-3415 (1993)


•Ueda, Y., Taguchi, S., Nishiyama, K., Kumagai, I. and Miura, K.*
Effect of a rare leucine codon, TTA, on expression of a foreign gene in Streptomyces lividans
Biochim. Biophys. Acta, 1172, 262-266 (1993)


•Nishiyama, K., Mizushima, S. and Tokuda, H.*
The carboxy-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli
J. Biol. Chem., 267, 7170-7176 (1992)


•Nishiyama, K., Kabuyama, Y., Akimaru, J., Matsuyama, S., Tokuda, H. and Mizushima, S.*
SecY is an indispensable component of the protein secretory machinery of Escherichia coli
Biochim. Biophys. Acta, 1065, 89-97 (1991)


•Tokuda, H., Akimaru, J. Matsuyama, S., Nishiyama, K. and Mizushima, S.*
Purification of SecE and reconstitution of SecE-dependent protein translocation activity
FEBS Lett., 279, 233-236 (1991)


•Taguchi, S., Nishiyama, K., Kumagai, I., Momose, H. and Miura, K.*
Relationship between utilization of dual translational initiation signals and protein processing in Streptomyces
Mol. Gen. Genet., 226, 328-331 (1991)


•Taguchi, S., Nishiyama, K., Kumagai, I. and Miura, K.*
Analysis of transcriptional control regions in the Streptomyces subtilisin-inhibitor-encoding gene
Gene, 84, 279-286 (1989)


•Suzuki, H., Nishiyama, K. and Tokuda, H.*
Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant
J. Biol. Chem., 274, 31020-31024 (1999)



総説・図書等

•西山賢一「タンパク質膜挿入・膜透過に関与する多機能性糖脂質MPIase」
生化学 88 (6): 744-747 (2016)

•Endo, Y. and Nishiyama, K. “Relationship between glycolipozyme MPIase and components comprising the protein transport machinery”
Med. Res. Arch. 2, paper no. 11 (2015)

•西山賢一、島本啓子「“糖脂質酵素(Glycolipozyme)” MPIaseの構造と作用機作」
酵素工学 74, 14-18 (2015)

•Nishiyama, K. and Shimamoto, K
Glycolipozyme Membrane Protein Integrase (MPIase): Recent Data
Biomol. Concepts, 5, 429-438 (2014)


•島本啓子、西山賢一
タンパク質膜挿入の鍵を握るグライコリポザイム~タンパク質でない酵素?~
実験医学増刊「代謝」、32, 115-122 (2014)


•島本啓子、西山賢一
膜タンパク質膜挿入の鍵を握る糖脂質酵素MPIase
生命化学研究レター、44, 9-14 (2014)


•西山賢一、島本啓子
膜タンパク質の鍵は糖脂質にあり―すべての生体膜挿入に必須な因子を求めて
月刊化学、68, 30-34 (2013)


•西山賢一
YidC
蛋白質 核酸 酵素(増刊「キーワード:蛋白質の一生」)、53, 1101 (2008)


•西山賢一
大腸菌における膜内在性タンパク質の膜挿入機構
生化学、79, 254-262 (2007)


•西山賢一
タンパク質輸送とRNA
RNA Network Newsletter, 5(1), 35-37 (2006)


•西山賢一
大腸菌の蛋白質膜挿入におけるYidCの機能
蛋白質 核酸 酵素、49, 959-960 (2004)


•西山賢一、遠藤斗志也
Meeting Report; EURESCO Conference on Protein-protein Interactions during Protein Translocation
LIFE OF PROTEINS, 13, 69-73 (2004)


•西山賢一
フライブルグ大学Matthias Muller研究室
LIFE OF PROTEINS, 11, 45-48 (2003)


•西山賢一
タンパク質膜透過を駆動するSecA-SecGの構造変化
分生研ニュース、16, 20 (2001)


•西山賢一
大腸菌における分泌タンパク質の膜透過分子機構 -膜透過を駆動するSecA-SecGの構造変化-
生化学、72, 1383-1397 (2000)


•西山賢一
分泌蛋白質の膜透過とSecA-SecGの構造変化
東京大学アイソトープ総合センターニュース、29(4), 4-6 (1999)


•西山賢一、徳田元
蛋白質膜透過装置の構造変化と作動機構
生物物理学会誌、38, 104-110 (1998)


•西山賢一、徳田元
1997(平成9年) 構造変化を繰り返す分泌蛋白質膜透過装置-膜透過反応に共役したSecGの膜内配向性反転-
蛋白質・核酸・酵素、42, 1-11 (1997)


•松山伸一、西山賢一、徳田元
分泌型蛋白質の細胞質膜透過実験法
生物化学実験法37、蛋白質の分泌と細胞内輸送、学会出版センター pp3-28 (1995)



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