原著論文

・Hikage, R., Tadika, Y., Asanuma, H., Han, Y., Nishiyama, K.,
"MucA is a small peptide encoded by an overlapping sequence with cdsA that upregulates the biosynthesis of glycolipid MPIase in the cold."
Biochem. Biophys. Res. Commun.,721, 150148 (2024)


・Hikage, R., Sekiya, Y., Sawasato, K., Nishiyama, K.,
"CdsA, a CDP-diacylglycerol synthase involved in phospholipid and glycolipid MPIase biosynthesis, possesses multiple initiation codons."
Genes Cells,29, 347-355 (2024)

・Kamemoto, Y., Hikage, R., Han, Y., Sekiya, Y., Sawasato, K., Nishiyama, K.,
"Coordinated upregulation of two CDP-diacylglycerol synthases, YnbB and CdsA, is essential for cell growth and membrane protein export in the cold."
FEMS Microbiol Lett.,370, 1-7(2023)

・Fujikawa, K.#, Han, Y.#, Osawa, T., Mori ,S., Nomura, K., Muramoto, M., Nishiyama, K., Shimamoto, K.,
"Structural requirements of a glycolipid MPIase for membrane protein integration"
Chem. Eur, J,29, e202300437 (2023) （#：first authors)

・Nomura, K., Mori, S., Fujikawa, K., Osawa, T., Tsuda, S., Yoshizawa-Kumagaye, K., Masuda, S.,
Nishio, H., Yoshiya, T., Yoda, T., Shionyu, M., Shirai, T., Nishiyama, K., & Shimamoto, K.,
"Role of a bacterial glycolipid in Sec-independent membrane protein insertion"
Sci. Rep. 12, 12231 (2022)

•Mori, S., Nomura, K., Fujikawa, K., Osawa, T., Shionyu, M., Yoda, T., Shirai, T., Tsuda, S.,
Yoshizawa-Kumagaya,K., Masuda, S., Nishio, H., Yoshiya, T., Suzuki, S., Muramoto, M.,
Nishiyama, K. Shimamoto, K.
"Intermolecular Interactions between aMembrane Protein and Glycolipid Essential for Membrane Protein integration"
ACS Chem. Biol,17, 609-618 (2022)

•Endo, Y., Shimizu, Y., Nishikawa, H., Sawasato, K., Nishiyama, K.,*
"Interplay between MPIase, YidC, and PMF during Sec-independent insertion of membrane proteins"
Life Sci. Alliance, 5, e202101162(2021)

•Sekiya, Y., Sawasato, K., Nishiyama, K.,*
"Expression of Cds4/5 of Arabidopsis chloroplasts in E coli reveals the membrane topology of the C-terminal region of CDP-diacylglycerol synthases"
Genes Cells,26, 727-738 (2021）

•Nishikawa, H., Kanno, K., Endo, Y., Nishiyama, K.*
"Ring assembly of c subunits of F0F1‐ATP synthase in Propionigenium modestum requires YidC and UncI following MPIase‐dependent membrane insertion"
FEBS Lett., 595, 647-654 (2021)

•Nishikawa, H., Sasaki, M., Nishiyama, K.*
"In vitro assay for bacterial membrane protein integration into proteoliposomes"
Bio-protoc., 10, e3626(2020)

•Saito, H., Morishita, T., Mizukami, T., Nishiyama, K., Kawaguchi, K., Nagao, H.
"Free energy profiles of lipid translocation across pure POPC and POPC/CHOL bilayer: all-atom molecular dynamics study"
J. Phys. Conf. Ser., 1290, 012020(2019)

•Sasaki, M., Nishikawa, H., Suzuki, S., Moser, M., Huber, M., Sawasato, K., Matsubayashi, H., Kumazaki, K., Tsukazaki, T., Kuruma, Y., Nureki, O., Ueda, T., Nishiyama, K.*
"The bacterial protein YidC accelerates MPIase-dependent integration of membrane proteins"
J. Biol. Chem., 294, 18898-18908 (2019)

•Nomura, K., Yamaguchi, T., Mori, S., Fujikawa, K., Nishiyama, K., Shimanouchi, T., Tanimoto, Y.,
Morigaki, K., Shimamoto, K.
"Alteration of membrane physicochemical properties by two facters for membrane protein integration"
Biophys. J.,117, 99–110 (2019)

•Kamemoto, Y., Funaba, N., Kawakami, M., Sawasato, K., Kanno, K., Suzuki, S., Nishikawa, H., Sato, R., Nishiyama, K.*
"Biosynthesis of glycolipid MPIase (membrane protein integrase) is independent of the genes for ECA (enterobacterial common antigen)"
J. Gen. Appl. Microbiol., 66, 169-174(2020)

•Sawasato, K., Sekiya, Y., Nishiyama, K.*
"Two-step induction of cdsA promoters leads to upregulation of the glycolipid MPIase at cold temperature."
FEBS Lett., 593, 1711–1723 (2019)
(selected as a "Editor's choice" paper)

•Sawasato, K., Suzuki, S., Nishiyama, K*.
"Increased expression of the bacterial glycolipid MPIase is required for efficient protein translocation across membranes in cold conditions."
J. Biol. Chem.,294, 8403-8411 (2019)

•Sato, R., Sawasato, K., Nishiyama, K.*
"YnbB is a CdsA paralogue dedicated to biosynthesis of glycolipid MPIase involved in membrane protein integration."
Biochem. Biophys. Res. Commun., 510, 636-642 (2019)

•Sawasato, K., Sato, R., Nishikawa, H., Iimura, N., Kamemoto, Y., Fujikawa, K., Yamaguchi, T., Kuruma, Y., Tamura, Y., Endo, T., Ueda, T., Shimamoto, K., Nishiyama, K.*
"CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo."
Sci. Rep., 9, 1372 (2019)

•Matsumura, K., Yamada, M., Yamashita, T., Muto, H., Nishiyama, K., Shimoi, H., Isobe, K.
"Expression of alcohol oxidase gene from Ochrobactrum sp. AIU 033 in recombinant Escherichia coli through the twin-arginine translocation pathway."
J. Biosci. Bioeng., 128, 13-21(2019)

•Saito, H., Morishita, T., Mizukami, T., Nishiyama, K., Kawaguchi,K., Nagao, H.
"Molecular dynamics study of binary POPC bilayers: molecular condensing effects on membrane structure and dynamics"
J. Phys., 1136, 012022(2018)

•Fujikawa, K. #, Suzuki, S #., Nagase, R., Ikeda, S., Mori, S., Nomura, K., Nishiyama, K*., Shimamoto, K*.
"Syntheses and activities of the functional structures of a glycolipid essential for membrane protein integration."
ACS Chem. Biol.,13, 2719-2727 (2018)　（#：first authors)

•Nakamura, S., Suzuki, S., Saito, H., Nishiyama, K. *,
"Cholesterol blocks spontaneous insertion of membrane proteins into liposomes of phosphatidylcholine."
J. Biochem., 163, 313-319 (2018)

•Nishikawa, H., Sasaki, M., Nishiyama, K. *,
"Membrane insertion of F0c subunit of F0F1 ATPase depends on glycolipozyme MPIase and is stimulated by YidC."
Biochem. Biophys. Res. Commun., 487, 477-482(2017)

•Nishiyama, K.* and Tokuda, H.
"Novel translocation intermediate allows re-evaluation of roles of ATP, proton motive force and SecG at the late stage of preprotein translocation”
Gene. Cells.,21, 1353-1364(2016)

•Kumazaki, K., Chiba, S., Takemoto, M., Furukawa, A., Nishiyama, K., Sugano, Y., Mori, T., Dohmae, N., Hirata, K., Nakada-Nakura, Y. Maturana, A.D., Tanaka, Y., Mori, H., Sugita, Y., Arisaka, F., Ito, K., Ishitani, R., Tsukazaki, T.*, Nureki, O. *
"Structural basis of Sec-independent membrane protein insertion by YidC"
Nature, 509, 516-520(2014)

•Moser, M., Nagamori, S. Huber, M., Tokuda, H. and Nishiyama, K. *
"Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation"
Proc. Natl. Acad. Sci. USA., 110, 9734-9739 (2013)

•Nishiyama, K.*, Maeda, M., Yanagisawa, K., Nagase, R., Komura, H., Iwashita, T., Yamagaki, T., Kusumoto, S., Tokuda, H. and Shimamoto, K.*
"MPIase is a glycolipozyme essential for membrane protein integration"
Nat. Commun., 3, 1260 (2012)

•Morita, K., Tokuda, H. and Nishiyama, K.*
"Multiple SecA molecules drive protein translocation across a single translocon with SecG inversion"
J. Biol. Chem.,287, 455-464(2012)

•Nishiyama, K.*, Maeda, M., Abe, M., Kanamori, T., Shimamoto, K., Kusumoto, S., Ueda, T. and Tokuda, H.
"A novel complete reconstitution system for membrane integration of the simplest membrane protein"
Biochem. Biophys. Res. Commun., 394, 733-736 (2010)

•Nishiyama, K.* and Tokuda, H. "
"Development of a functional in vitro integration system for an integral membrane protein, SecG"
Biochem. Biophys. Res. Commun., 390, 920-924 (2009)

•Antonoaea, R., Furst, M., Nishiyama, K. and Muller, M.*
"The periplasmic chaperone PpiD interacts with secretory proteins exiting from the SecYEG translocon"
Biochemistry, 47, 5649-5656 (2008)

•Kawashima, Y., Miyazaki, E., Muller, M., Tokuda, H. and Nishiyama, K.*
"Diacylglycerol specifically blocks spontaneous integration of membrane proteins and allows detection of a factor-assisted integration"
J. Biol. Chem., 283, 24489-24496 (2008)

•Sugai, R., Takemae, K., Tokuda, H. and Nishiyama, K.*
"Topology inversion of SecG is essential for cytosolic SecA-dependent stimulation of protein translocation"
J. Biol. Chem., 282, 29540-29548 (2007)

•Nishiyama, K.*, Ikegami, A., Moser, M., Schiltz, E., Tokuda, H. and Muller, M.*
"A derivative of lipid A is involved in signal recognition particle/SecYEG-dependent and -independent membrane integrations"
J. Biol. Chem., 281, 35667-35676 (2006)

•Nishiyama, K.* and Tokuda, H.
"Genes coding for SecG and Leu2-tRNA form an operon to give an unusual RNA comprising mRNA and a tRNA precursor"
Biochim. Biophys. Acta, 1729, 166-173 (2005)

•Ikegami A., Nishiyama K., Matsuyama S. and Tokuda H.*
"Disruption of rpmJ encoding ribosomal protein L36 decreases the expression of secY upstream of the spc operon and inhibits protein translocation in Escherichia coli"
Biosci. Biotechnol. Biochem., 69, 1595-1602 (2005)

•Kuruma, Y., Nishiyama, K., Shimizu, Y., Muller, M. and Ueda, T.*
"Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins"
Biotechnol. Prog., 21, 1243-1251 (2005)

•Sugai, R., Shimizu, H., Nishiyama, K. and Tokuda, H.*
"Overexpression of gnsA, a multicopy suppressor of the secG null mutation, increases acidic phospholipid contents by inhibiting phosphatidylethanolamine synthesis at low temperatures"
J. Bacteriol.,186, 5968-5971 (2004)

•Kato, Y., Nishiyama, K. and Tokuda, H.*
"Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction"
FEBS Lett., 550, 114-118 (2003)

•Nagamori, S., Nishiyama, K. and Tokuda, H.*
"Membrane topology inversion of SecG detected by labeling with a membrane-impermeable sulfhydryl reagent that causes a close association of SecG with SecA"
J. Biochem.,132, 629-634 (2002)

•Sugai, R., Shimizu, H., Nishiyama, K. and Tokuda, H.*
"Overexpression of yccL (gnsA) and ydfY (gnsB) increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation of Escherichia coli"
J. Bacteriol., 183, 5523-5528 (2001)

•Nagamori, S., Nishiyama, K. and Tokuda, H.*
"Two SecG molecules present in a single protein translocation machinery are functional even after crosslinking"
J. Biochem., 128, 129-137 (2000)
(This paper received the JB award.)

•Nishiyama, K., Suzuki, H. and Tokuda, H.*
"Role of the non-essential region encompassing the N-terminal two transmembrane stretches of Escherichia coli SecE"
Biosci. Biotechnol. Biochem., 64, 2121-2127 (2000)

•Nishiyama, K., Fukuda, A., Morita, K. and Tokuda, H.*
"Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation"
EMBO J., 18, 1049-1058 (1999)

•Suzuki, H., Nishiyama, K. and Tokuda, H.*
"Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant"
J. Biol. Chem., 274, 31020-31024 (1999)

•Nishiyama, K., Furuta, M. and Tokuda, H*
"Molecular cloning and functional characterization of SecE of a marine bacterium, Vibrio alginolyticus"
Biochem. Biophys. Res. Commun., 251, 894-897 (1998)

•Suzuki, H., Nishiyama, K. and Tokuda, H.*
"Coupled structure change of SecA and SecG revealed by the synthetic lethality of the secAcsR11 and ΔsecG::kan double mutant"
Mol. Microbiol., 29, 331-341 (1998)

•Shimizu, H., Nishiyama, K. and Tokuda, H.*
"Expression of gpsA encoding biosynthetic sn-glycerol 3-phosphate dehydrogenase suppresses both the LB- phenotype of a secB null mutant and the cold-sensitive phenotype of a secG null mutant"
Mol. Microbiol.,26, 1013-1021(1997)

•Kontinen, V.P., Yamanaka, M., Nishiyama, K. and Tokuda, H.*
"Roles of the conserved cytoplasmic region and non-conserved carboxy-terminal region of SecE in Escherichia coli protein translocase"
J. Biochem., 119, 1124-1130 (1996)

•Nishiyama, K., Suzuki, T. and Tokuda, H.*
"Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation"
Cell, 85, 71-81 (1996)

•Hanada, M., Nishiyama, K. and Tokuda, H.*
"SecG plays a critical role in protein translocation in the absence of the proton motive force as well as at low temperature"
FEBS Lett., 381, 25-28 (1996)

•Nishiyama, K., Mizushima, S. and Tokuda, H.*
"Preferential interaction of SecG with SecE stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane"
Biochem. Biophys. Res. Commun., 217, 217-223 (1995)

•Hanada, M., Nishiyama, K., Mizushima, S. and Tokuda, H.*
"Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12)"
J. Biol. Chem.,269, 23625-23631 (1994)

•Douville, K., Leonard, M., Brundage, L., Nishiyama, K., Tokuda, H., Mizushima, S. and Wickner, W.*
"Band 1 subunit of Escherichia coli preprotein translocase and integral membrane export factor p12 are the same protein"
J. Biol. Chem., 269, 18705-18707 (1994)

•Nishiyama, K., Hanada, M. and Tokuda, H.*
"Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature"
EMBO J, 13, 3272-3277 (1994)

•Nishiyama, K., Mizushima, S. and Tokuda, H.*
"A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli"
EMBO J., 12, 3409-3415 (1993)

•Ueda, Y., Taguchi, S., Nishiyama, K., Kumagai, I. and Miura, K.*
"Effect of a rare leucine codon, TTA, on expression of a foreign gene in Streptomyces lividans"
Biochim. Biophys. Acta, 1172, 262-266 (1993)

•Nishiyama, K., Mizushima, S. and Tokuda, H.*
"The carboxy-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli"
J. Biol. Chem., 267, 7170-7176 (1992)

•Nishiyama, K., Kabuyama, Y., Akimaru, J., Matsuyama, S., Tokuda, H. and Mizushima, S.*
"SecY is an indispensable component of the protein secretory machinery of Escherichia coli"
Biochim. Biophys. Acta, 1065, 89-97 (1991)

•Tokuda, H., Akimaru, J. Matsuyama, S., Nishiyama, K. and Mizushima, S.*
"Purification of SecE and reconstitution of SecE-dependent protein translocation activity"
FEBS Lett., 279, 233-236(1991)

•Taguchi, S., Nishiyama, K., Kumagai, I., Momose, H. and Miura, K.*
"Relationship between utilization of dual translational initiation signals and protein processing in Streptomyces"
Mol. Gen. Genet.,226, 328-331 (1991)

•Taguchi, S., Nishiyama, K., Kumagai, I. and Miura, K.*
"Analysis of transcriptional control regions in the Streptomyces subtilisin-inhibitor-encoding gene"
Gene, 84, 279-286 (1989)

総説・図書等

・Shimamoto, K., Fujikawa, K., Osawa, T., Mori, S., Nomura, K., Nishiyama, K.,
"Key contributions of a glycolipid to membrane protein integration"
Proc. Jpn. Acad., ser. B, 100, 387-413 (2024)


・Fujikawa, K., Mori, S., Nishiyama, K., Shimamoto, K.,
"Chapter Five - A bacterial glycolipid essential for membrane protein integration"
ACCB,81, 95-129 (2022)

・Nishikawa, H., Sawasato, K., Mori, S., Fujikawa, K., Nomura, K., Shimamoto,K., and Nishiyama, K.,*
"Interaction between glycolipid MPIase and proteinaceous factors during protein integration into the cytoplasmic membrane of E. coli"
Front. Mol. Biosci ., 9, 986602 (2022)

•沢里克宏、藤川絋樹、島本啓子、西山賢一
「大腸菌におけるタンパク質膜輸送に関与する糖脂質MPIaseの発現制御機構～酵素様機能をもつ糖脂質MPIase～」
化学と生物 58, 223-230 (2020)

•Fujikawa,K., Nomura,K., Nishiyama,K., Shimamoto, K.
"Novel Glycolipid Involved in Membrane Protein Integration: Structure and Mode of Action"
J. Synth. Org. Chem. Jpn.,77, 1096-1105 (2019)

•Fujikawa, K., Nishiyama, K., Shimamoto, K.
"Enzyme-like Glycolipids MPIase Involved in Membrane Protein Integration of E. coli."
Trends in Glycoscience and Glycotechnology, 31, E151-E158 (2019)

•藤川 紘樹、 西山 賢一、 島本 啓子　
「大腸菌膜タンパク質の膜挿入に関与する酵素様糖脂質MPIase」
Trends in Glycoscience and Glycotechnology, 31, J149-J155 (2019)

•西山賢一
「タンパク質膜挿入・膜透過に関与する多機能性糖脂質MPIase」
生化学 88 (6): 744-747 (2016)

•Endo, Y. and Nishiyama, K.
"Relationship between glycolipozyme MPIase and components comprising the protein transport machinery”
Med. Res. Arch.2, paper no. 11 (2015)

•西山賢一、島本啓子「“糖脂質酵素（Glycolipozyme）” MPIaseの構造と作用機作」
酵素工学 74, 14-18 (2015)

•Nishiyama, K. and Shimamoto, K
"Glycolipozyme Membrane Protein Integrase (MPIase): Recent Data"
Biomol. Concepts, 5, 429-438 (2014)

•島本啓子、西山賢一
タンパク質膜挿入の鍵を握るグライコリポザイム～タンパク質でない酵素？～
実験医学増刊「代謝」、32, 115-122 (2014)

•島本啓子、西山賢一
膜タンパク質膜挿入の鍵を握る糖脂質酵素MPIase
生命化学研究レター、44, 9-14 (2014)

•西山賢一、島本啓子
「膜タンパク質の鍵は糖脂質にあり―すべての生体膜挿入に必須な因子を求めて」
月刊化学、68, 30-34 (2013)

•西山賢一
「YidC」
蛋白質 核酸 酵素（増刊「キーワード：蛋白質の一生」）、53, 1101 (2008)

•西山賢一
「大腸菌における膜内在性タンパク質の膜挿入機構」
生化学、79, 254-262 (2007)

•西山賢一
「タンパク質輸送とRNA」
RNA Network Newsletter, 5(1), 35-37 (2006)

•西山賢一
「大腸菌の蛋白質膜挿入におけるYidCの機能」
蛋白質 核酸 酵素、49, 959-960 (2004)

•西山賢一、遠藤斗志也
"Meeting Report; EURESCO Conference on Protein-protein Interactions during Protein Translocation"
LIFE OF PROTEINS, 13, 69-73 (2004)

•西山賢一
「フライブルグ大学Matthias Muller研究室」
LIFE OF PROTEINS, 11, 45-48 (2003)

•西山賢一
「タンパク質膜透過を駆動するSecA-SecGの構造変化」
分生研ニュース、16, 20 (2001)

•西山賢一
「大腸菌における分泌タンパク質の膜透過分子機構 －膜透過を駆動するSecA-SecGの構造変化－」
生化学、72, 1383-1397 (2000)

•西山賢一
「 分泌蛋白質の膜透過とSecA-SecGの構造変化」
東京大学アイソトープ総合センターニュース、29(4), 4-6 (1999)

•西山賢一、徳田元
「 蛋白質膜透過装置の構造変化と作動機構」
生物物理学会誌、38, 104-110 (1998)

•西山賢一、徳田元
「1997（平成９年) 構造変化を繰り返す分泌蛋白質膜透過装置－膜透過反応に共役したSecGの膜内配向性反転－」
蛋白質・核酸・酵素、42, 1-11 (1997)

•松山伸一、西山賢一、徳田元
「 分泌型蛋白質の細胞質膜透過実験法」
生物化学実験法37、蛋白質の分泌と細胞内輸送、学会出版センター pp3-28 (1995)